GFP Nanoselector Magnetic beads

Description: 

GFP Nanoselector Magnetic beads have been specifically designed to bind GFP-fusion proteins. GFP Nanoselector Magnetic beads is based on small high-affinity recombinant alpaca antibody fragments covalently coupled to the surface of Magnetic beads. GFP Nanoselector Magnetic beads are ideal tools to isolate or purify GFP-fusion proteins fast and efficiently.
 

Ligand: Anti-GFP single domain antibody fragment (VHH, Nanobody)
Bead size: ~ 40 µm

Reactivity: Recognizes GFP, mEGFP, superfolder GFP and most common CFP and YFP variants. Does not cross-react with mCherry, mRFP, dsRed, mTagBFP, mTagRFP or their most common derivatives.
Binding capacity: High binding capacity, 10 µL slurry bind about 20 µg of recombinant GFP.
Storage: Shipped at ambient temperature. Upon receipt store at 4°C. Stable for 1 year. Do not freeze.
Storage Buffer: 10mM PBS (pH 7.5), 0.01% proclin300

Background:

Green fluorescent proteins (GFPs) and the variants thereof are widely used to study protein localization and dynamics. For biochemical analysis including mass spectrometry and enzyme activity measurements these GFP-fusion proteins and their interacting factors need to be isolated fast and efficiently by immunoprecipitation using the GFP Nanoselector Magnetic beads. Due to the single-chain nature of sdAbs and their stable and covalent attachment, no leakage of light and heavy chains is observed during elution with SDS sample buffer.